Delstaphinine [1], a new C₁₉-diterpenoid alkaloid, and 1-dehydrodelphisine have been isolated from the seeds of Delphinium staphisagria. The structures have been determined with the aid of ¹H- and ¹³C-nmr spectroscopy and were confirmed by synthesis from neoline and delphisine, respectively. 1-Dehydrodelphisine has not been isolated from a natural source previously. The seeds of Delphinium staphisagria L. (Ranunculaceae), when extracted with ligroin, yield an alkaloid fraction of which delphinine (1) is the major component. The mother liquor accumulated during the isolation of a large quantity of delphinine furnished an amorphous fraction from which delphisine (2), delphidine (3), delphirine (epineoline) (4), and several bisditerpenoid alkaloids have been isolated (5-7). Recently we reported the isolation of three new alkaloids, delstaphisine, delstaphisagrine, and delstaphisagnine from this amorphous fraction (8). In this paper we report separation of an amorphous fraction from the seeds of D. staphisagria by a combination of gradient pH separation, vacuum liquid chromatography (vlc), preparative tlc, and centrifugally accelerated, radial, tlc ("Chromatotron") to give a new C₁₉-diterpenoid alkaloid, delstaphinine [1], and the alkaloid, 1-dehydrodelphisine [7]. The latter has not been reported in nature before but had been prepared by CrO₃ oxidation of delphisine (2).