Three new C₁₉-diterpenoid alkaloids, delstaphisine (3), delstaphisagria (4) and delstaphisagnine (5) have been isolated from the seeds of Delphinium staphisagria by a combination of gradient pH extractions and chromatographic techniques, including vacuum liquid chromatography (vlc) and centrifugally accelerated, radial, thin-layer chromatography ("Chromatotron"). The structures have been determined with the aid of proton and carbon-13 nmr spectroscopy. Delstaphisine (3) is the first reported aconitine-type, C₁₉-diterpenoid alkaloid bearing a C(16)-hydroxyl group.