Alkaloids of three species ofBerberis

Chemistry of Natural Compounds
1976.0

Abstract

We studied the alkaloids in the leaves of three Berberis species (B. oblonga, B. intergerrima, B. nummularia) and Ammopipanthus mongolicus. For the three Berberis species: B. oblonga (collected in Tashkent oblast in May 1975, flowering phase) had 0.04% total bases; extraction with chloroform and methanol, separation on silica gel yielded thalicmidine, isocorydine, and a small amount of berberine, with qualitative differences from root alkaloids. B. intergerrima (collected in Kirghizia in July 1974, fruit-bearing phase) had 0.18% total alkaloids; four bases were isolated from the nonphenolic fraction, identified as isocorydine, thalicmidine, glaucine, and hydroxyacanthine, plus berberine; main alkaloids were thalicmidine and glaucine. B. nummularia (collected in Chimgan in May 1975, flowering phase) had 0.052% total alkaloids, yielding thalicmidine and isocorydine. The main alkaloids in the three species were aporphine type; thalicmidine and isocorydine were first isolated from Berberis genus. For Ammopipanthus mongolicus (collected in September 1974 in Ala-Shan Gobi, Mongolia), chloroform extraction gave 2.5% of base mixture; repeated separation by basicity, silica gel column chromatography, and salt preparation yielded nine individual alkaloids. About half the total was sparteine (I, isolated as perchlorate with mp 171-172°C, [α]D -12.5°); 10% was d-lupanine (II, isolated as hydriodide with mp 189-190°C, [α]D +44°); base III (mp 105-110°C, [α]D -67°) was identified as α-isosparteine; bases IV-VI were in very small amounts.

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