The genus Cephalotaxus (Cephalotaxaceae) has long been known to contain the antileukemic ester alkaloids harringtonine and its congeners. Although some oxygenated Cephalotaxus alkaloids, such as 11-hydroxycephalotaxine and drupacine, have been isolated, there has been no report of the isolation of oxygenated ester-type congeners. Three oxygenated cephalotaxine ester-type alkaloids, 11R-hydroxyhomodeoxyharringtonine (1), 11β-hydroxyhomodeoxyharringtonine (2) and 11β-hydroxydeoxyharringtonine (3) were isolated from Cephalotaxus harringtonia var. drupacea. Their structures were established by spectroscopic methods including 2D NMR and CD spectra, and their antileukemic activity was evaluated using P-388 leukemia cells. We report herein the isolation, characterization, and antileukemic activities of these alkaloids against P-388 leukemia cells.