A new alkaloid, which has been called karakanine, has been isolated from the total alkaloids of the epigeal part of Aconitum karakolicum. The structure of karakanine as 8β,14α-dihydroxy-16β-methoxy-4β-methyl-N-ethyl-1α,19α-epoxyaconitane has been proposed on the basis of a study of its IR, PMR, mass, and13C NMR spectra, and has been confirmed by its conversion into karakoline. © 1993 Plenum Publishing Corporation.