We have investigated the tubers of Aconitum karakolicum collected from two growth sites in the period of the withering of the epigeal part. From a sample collected in the upper reaches of the R. Tyup, Terskei-Alatau range of the Kirghiz SSR, in addition to aconitine and songorine found previously, we have isolated napelline and a new alkaloid C34H47O12N called aconifine (I); aconifine contains a N-ethyl group, four methoxy groups, a benzoyl group, an acetyl group, and four hydroxy groups, has a mass spectrum characteristic for alkaloids with the lycoctonine skeleton similar to aconitine, and its partial structure was proposed based on NMR and mass spectral data. From the tubers collected in the Kirghiz SSR (Kungei-Alatau range), we have isolated songorine, karakoline, napelline, and a new alkaloid C22H33O5N called karacolidine (II); karacolidine contains N-ethyl, tertiary C-methyl, methoxy, and four hydroxy groups, has a mass spectrum corresponding to alkaloids with the lycoctonine skeleton, and its structure was determined based on acetylation, oxidation, pyrolysis, and spectral data.