A novel Cig-diterpenoid alkaloid, karaconltine (1) and four known alkaloids, 3-deoxyaconitine (2), aconitine (3), indaconitine (4) and chasmaconitine (5), have been isolated from the roots of Aconitum karakolicum Rap. The structure of karaconitine (1) was determined to be 2α-hydroxy-3-deoxyaconitine by the analyses of its spectral data.