Aconitum barbatum var. puberulum is widely used in Chinese folk medicine for the treatment of rheumatosis, rheumatoid arthritis, and some other inflammations [1]. In our phytochemical investigation of traditional Chinese medicines, an aporphine alkaloid corydine (1) [2], a tricarboxylic unsaturated acid, aconitic acid (3) [3], and two diterpenoid alkaloids, tuguaconitine (2) [4] and luciculine (4) [5], were isolated and identified. These compounds were isolated from this plant for the first time. The 13C NMR of compound 1 was reported for the first time, and the 13C NMR of compounds 2 and 4 were reassigned.