Nine diterpenoid alkaloids were isolated from the roots of ACONITUM BARBATUM VAR. PUBERULUM. Five of them are known: ranaconitine ( 1) [1], lappaconitine ( 2) [2], septentriodine ( 3) [3], septentrionine ( 4) [3], and lycaconitine ( 5) [4]. The other four are new: puberanine ( 6), puberanidine ( 7), puberaconitine ( 8) and puberaconitidine ( 9).