From the aerial parts of Solanum dulcamara, two spirostanol glycosides (1 and 2) and two new spirosolane glycosides (3 and 4) were isolated and their chemical structures were characterized as tigogenin 3-O-β-solatrioside, degalactotigonin, soladulcidine 3-O-β-chacotrioside (named soladulcine A), and soladulcidine 3-O-β-lycotetraoside (named soladulcine B), respectively.