We report the isolation and characterization of a new aporphine alkaloid, hernagine, and a pyridine alkaloid, 3-cyano-4-methoxypyridine, from the leaves of Hernandia nymphaefolia, along with known aporphine alkaloids (nornantenine, laurotetanine, N-methyllaurotetanine, oxonantenine, ovigerine, nandigerine, isocorydine) from the bark of the same species. Hernagine's structure (1) was determined via ¹H NMR, MS, UV spectroscopy, and chemical conversion to N-methylhernagine (2). 3-Cyano-4-methoxypyridine (11), isolated from both phenolic and non-phenolic fractions, was identified by spectroscopic data and comparison with synthetic material, representing a new natural product. Additionally, mayfoline, a novel alkaloid with a hydroxylamine moiety, was isolated from the aerial parts of Maytenus buxifolia; its structure (1) was established using IR, UV, ¹H NMR, MS, and acetylation to N,O-diacetate (2), and it belongs to macrocyclic spermidine alkaloids found in other Maytenus and Celastraceae species.