Coronilla varia suspension cultures incorporated exogenous 3-nitropropanoic acid into 3-nitropropanoyl-D-glucopyranoses, increasing their levels 600-fold over control values. The resulting levels of 3-nitropropanoyl-D-glucopyranoses were still lower than those in C. varia plants but were high enough for isolation of hiptagin (1,2,4,6-tetra(3-nitropropanoyl)-β-D-glucopyranose), coronarian and cibarian. Structural assignments were partially made on the basis of 360 MHz 1H NMR spectra.