Further investigation of the active principles of Zygadenus venenosus has led to the isolation of the hypotensive germine esters neogermitrine, germidine, protoveratridine and a new diester isomeric with germidine, for which we have proposed the name neogermidine. Neogermidine, like germidine, is a monoacetate mono-(l)-α-methylbutyrate of the alkamine germine. Upon acetylation with acetic anhydride and pyridine, neogermidine is converted to monoacetylneogermitrine.