In the previous investigation on the free amino acid contents in the mycelium of Er. ashbyii, hydroxykynurenine was (+) and proline was (-) in the yellow-type strain and hydroxykynurenine was (-) and proline was (+) in the leuco-type strain. This time the investigation was reexamined with a little different result that hydroxykynurenine was (+) and proline was (+) in the yellow-type strain and hydroxykynurenine was (-) and proline was (+) in the leuco-type strain. Consequently the amino acids were examined more minutely using automatic amino acid analyzer, and from the results shown in the table it was concluded that the characterization of the two strains by the specific amino acids contained in them is unreasonable.