<jats:title>ABSTRACT</jats:title> <jats:p> <jats:italic>Streptomyces gilvosporeus</jats:italic> strain F607 is a producer of high levels of natamycin used in the fermentation industry. In this study, the complete genome sequence of strain F607 was determined. This genome sequence provides a basis for understanding natamycin biosynthesis and regulation in a high-natamycin-producing strain and will aid in the development of useful strategies for improving industrial strains.