Alkaloids of pedicularis

Chemistry of Natural Compounds
1969.0

Abstract

From the epigeal part of P. rhinanthoides Schrenk., collected on July 21, 1966 in the flowering period in the gorge of the R. Nura, KirgSSR, 0.3% of total alkaloids were obtained by chloroform extraction; the ethereal fraction treated with acetone gave 0.04% plantagonine, and the mother liquor separated on an alumina column (eluant: ether-chloroform 9:1) yielded a liquid base identified as the dextrorotatory form of 1-tecostidine. From the epigeal part of P. olgae Rgl., collected on June 15, 1968 in the flowering period in Saed village, TadzhSSR, 0.65% total alkaloids were obtained, from which 0.11% plantagonine was isolated. From the seeds of cotton variety 10S-F, a fraction with malate dehydrogenase activity was isolated; the process included defatting the ground seed coats, extracting with 0.01 M phosphate buffer (pH 7.4) containing EDTA and β-mercaptoethanol, centrifugation, ammonium sulfate fractional precipitation (20%-50% saturation), dissolving the precipitate, and passing through a Sephadex G-25 column.

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