Bromination of the purine alkaloid triacanthine by N-bromosuccinimide in CHCl3, AcOH, and various alcohols was studied. The new derivatives 8-bromo-, 12,13-dibromo-, 8,12-dibromo-13-hydroxymethyl-, hydroxyethyl-, hydroxypropyl-, and hydroxybutyltriacanthine were prepared. The structures of the prepared compounds were proven using H-1 and C-13 NMR spectral data and X-ray crystal structure analyses.