A new C18-diterpenoid alkaloid, hispaconitine (1) and four known alkaloids, tuguaconitine (2), delsoline (3), 14-acetyldelcosine (4) and delcosine (5), have been isolated from the roots of Aconitum barbetum var. hispidum Ledeb. The structure of hispaconitine (1) was determined by 2D-nmr spectroscopic analyses. © 1990.