Analogs of hygromycin A in which the aminocyclitol is replaced by dihydroxycyclohexylamine and trihydroxycyclohexylamine (with stereochemistry matching that of the natural product) have been prepared. The latter was prepared both in racemic form (starting with 1,3-cyclohexadiene) and as a single enantiomer (by degradation of hygromycin A.) Antibacterial activities against key animal health pathogens are reported.