This study examined the flavonoid chemosystematics of two Verbena species (V. ozcinalis and V. supina) from Egypt. Dried plant materials were extracted with 70% ethanol, fractionated on polyamide columns using water and increasing concentrations of ethanol, and further purified via Whatman No. 3MM paper chromatography and Sephadex LH-20 columns. The structures of isolated flavonoids were elucidated using standard methods—complete/mild acid hydrolysis, enzymatic hydrolysis, co-chromatography with authentic samples, and UV spectroscopy—with H¹-NMR confirming two uncommon flavonoids (luteolin-7-neohesperidoside and diosmetin-7-neohesperidoside). Eight flavonoid glycosides were identified: apigenin-7-glucoside, luteolin-7-glucoside, luteolin-7-galactoside, luteolin-7-neohesperidoside, diosmetin-7-glucoside, diosmetin-7-galactoside, diosmetin-7-neohesperidoside, and chrysoeriol-7-galactoside. Distribution analysis showed that luteolin-7-neohesperidoside and diosmetin-7-neohesperidoside were major components in V. ozcinalis, while diosmetin-7-glucoside was the major component in V. supina. Both species contained trace amounts of apigenin-7-glucoside and chrysoeriol-7-galactoside, as well as varying levels of luteolin-7-glucoside, luteolin-7-galactoside, and diosmetin-7-galactoside. These flavonoid profiles support the classification of the two taxa as distinct species, with only minor quantitative differences between fresh and herbarium samples.