We have already reported the isolation and characterization of berberine chloride, palmatine iodide and berbamine from the roots of Berberis lycium Royle. In this study, we describe the isolation and characterization of two artefact alkaloids—berberine-CHCl₃ (I, berberinechloroform, previously reported by Gaze) and palmatine-CHCl₃ (II, palmatinechloroform, first reported herein)—and oxyberberine (III). Alkaloid I was identified by its molecular formula C₂₁H₁₈NO₄Cl₃ (with chlorine content 23.59%), UV spectrum (characteristic of dihydroprotoberberines) and NMR spectrum (singlet at 4.34 τ for the C-8 proton). Alkaloid II, with molecular formula C₂₂H₂₂NO₄Cl₃, was characterized by its UV spectrum (dihydroprotoberberine characteristic) and NMR spectrum (four methoxyl groups and a singlet at 4.36 τ for the C-8 proton), representing the first report of this artefact. Alkaloid III, C₂₀H₁₇NO₅, was confirmed as oxyberberine by comparison with an authentic sample (melting point, mixed melting point and IR spectrum) and its spectral data: IR with a 1650 cm⁻¹ amide band, UV characteristic of dihydroprotoberberines, and NMR showing peaks for methylene, methoxyl, methylenedioxy and aromatic protons.