A new flavone, 3′-hydroxy-5,6,7,4′,5′-pentamethoxyflavone (1), and three natural products (7, 10, and 14) wereisolated from the leaves and twigs of Murraya paniculata (L.) Jack, together with 10 reported polymethoxylatedflavonoids. The structure of compound 1 was elucidated on the basis of comprehensive analysis of 1D and 2DNMR and HRMS spectroscopic data. Moreover, the chemotaxonomic significance of these compounds was alsodiscussed.