The new base acutinine and the known alkaloids skimmianine, evoxine, glycoperine, perfamine, and haplamine were isolated from total alkaloids from the aerial part of Haplophyllum acutifolium collected in Jizzakh Oblast, Republic of Uzbekistan. Acutinine was identified as a quinolin-2-one derivative based on spectral characteristics. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.