Alkaloids from the aerial part, seeds, and roots of the plant Haplophyllum perforatum collected in Kashkadarya Region, Republic of Uzbekistan, were studied. The new quinoline alkaloid perfirine in addition to known alkaloids (evoxine, skimmianine, glycoperine, methylevoxine, evodine, flindersine, haplamine, dictamnine, robustine, perfamine) were isolated from the seeds. Perfirine was a modified furanoquinoline derivative based on IR, UV, and NMR spectral data. © 2022, Springer Science+Business Media, LLC, part of Springer Nature.